期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 114, 期 -, 页码 10-18出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2017.02.015
关键词
Cadmium stress; Chlorophyll fluorescence; DELLA proteins; P700 photooxidation; Photochemical activity; Rht-B1c mutant
资金
- Bulgarian Academy of Sciences
- Republic of Bulgaria [01/10]
- Slovak Republic [01/10]
The sensitivity to cadmium (Cd) stress of two near-isogenic wheat lines with differences at the Rht-B1 locus, Rht-B1a (tall wild type, encoding DELLA proteins) and Rht-B1c (dwarf mutant, encoding modified DELLA proteins), was investigated. The effects of 100 mu M CdCl2 on plant growth, pigment content and functional activity of the photosynthetic apparatus of wheat seedlings grown on a nutrient solution were evaluated through a combination of PAM chlorophyll fluorescence, oxygen evolution, oxidation-reduction kinetics of P700 and 77 K fluorescence. The results showed that the wheat mutant (Rht-B1c) was more tolerant to Cd stress compared to the wild type (Rht-B1a), as evidenced by the lower reductions in plant growth and pigment content, lower inhibition of photosystem I (PSI) and photosystem II (PSII) photochemistry and of the oxygen evolution measured with Clark-type and Joliot-type electrodes. Furthermore, the enhanced Cd tolerance was accompanied by increased Cd accumulation within mutant plant tissues. The molecular mechanisms through which the Rht-B1c mutation improves plant tolerance to Cd stress involve structural alterations in the mutant photosynthetic membranes leading to better protection of the Mn cluster of oxygen-evolving complex and increased capacity for PSI cyclic electron transport, protecting photochemical activity of the photosynthetic apparatus under stress. This study suggests a role for the Rht-B1c-encoded DELLA proteins in protective mechanisms and tolerance of the photosynthetic apparatus in wheat plants exposed to heavy metals stress. (C) 2017 Elsevier Masson SAS. All rights reserved.
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