期刊
PLANT PHYSIOLOGY
卷 175, 期 3, 页码 1269-1282出版社
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.17.01187
关键词
-
资金
- Italian Ministry of Education (Progetti di Rilevanza Nazionale, PRIN)
- Italian Ministry of Education (PRO-ROOT) [20105XLAXM]
- Rete delle Biotecnologie in Campania, Progetto Bio Industrial Processes - BIP [CUP B25C13000290007]
- Institut National de la Recherche Agronomique
- Agence Nationale de la Recherche [ANR-14-CE34-0007-01-HONIT]
- European Molecular Biology Organization
N-fixing nodules are new organs formed on legume roots as a result of the beneficial interaction with soil bacteria, rhizobia. The nodule functioning is still a poorly characterized step of the symbiotic interaction, as only a few of the genes induced in N-fixing nodules have been functionally characterized. We present here the characterization of a member of the Lotus japonicus nitrate transporter1/peptide transporter family, LjNPF8.6. The phenotypic characterization carried out in independent L. japonicus LORE1 insertion lines indicates a positive role of LjNPF8.6 on nodule functioning, as knockout mutants display N-fixation deficiency (25%) and increased nodular superoxide content. The partially compromised nodule functioning induces two striking phenotypes: anthocyanin accumulation already displayed 4 weeks after inoculation and shoot biomass deficiency, which is detected by long-term phenotyping. LjNPF8.6 achieves nitrate uptake in Xenopus laevis oocytes at both 0.5 and 30 mM external concentrations, and a possible role as a nitrate transporter in the control of N-fixing nodule activity is discussed
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据