期刊
PLANT JOURNAL
卷 91, 期 4, 页码 714-724出版社
WILEY
DOI: 10.1111/tpj.13599
关键词
genome editing; CRISPR/Cas9; hexaploid wheat; Triticum aestivum L.; powdery mildew; Blumeria graminis; EDR1
资金
- National Science Fund for Distinguished Young Scholars of China [31525019]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB11020100]
- Ministry of Science and Technology of China [2015CB910202, 2014DFA31540]
- National Key Research and Development Program of China [2016YFD0101804]
- Chinese Academy of Sciences [KFZD-SW-107]
Wheat (Triticum aestivum L.) incurs significant yield losses from powdery mildew, a major fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). enhanced disease resistance1 (EDR1) plays a negative role in the defense response against powdery mildew in Arabidopsis thaliana; however, the edr1 mutant does not show constitutively activated defense responses. This makes EDR1 an ideal target for approaches using new genome-editing tools to improve resistance to powdery mildew. We cloned TaEDR1 from hexaploid wheat and found high similarity among the three homoeologs of EDR1. Knock-down of TaEDR1 by virus-induced gene silencing or RNA interference enhanced resistance to powdery mildew, indicating that TaEDR1 negatively regulates powdery mildew resistance in wheat. We used CRISPR/Cas9 technology to generate Taedr1 wheat plants by simultaneous modification of the three homoeologs of wheat EDR1. No off-target mutations were detected in the Taedr1 mutant plants. The Taedr1 plants were resistant to powdery mildew and did not show mildew-induced cell death. Our study represents the successful generation of a potentially valuable trait using genome-editing technology in wheat and provides germplasm for disease resistance breeding.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据