期刊
PLANT JOURNAL
卷 92, 期 1, 页码 19-30出版社
WILEY
DOI: 10.1111/tpj.13630
关键词
Ananas comosus; CAM photosynthesis; circadian regulation; cis-element; stomata
资金
- Department of Science and Technology of Fujian Province [2016NZ0001-1]
- National Science Foundation [DBI-1401572]
- Department of Energy, Office of Science, Genomic Science Program [DE-SC0008834]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1401572] Funding Source: National Science Foundation
- Office Of The Director
- Office of Integrative Activities [1355406] Funding Source: National Science Foundation
The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C-3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and microRNA (miRNA) expression patterns in the obligate CAM plant pineapple [Ananas comosus (L.) Merr.]. The high-resolution transcriptome atlas allowed us to distinguish between CAM-related and non-CAM gene copies. A differential gene co-expression network across green and white leaf diel datasets identified genes with circadian oscillation, CAM-related functions, and source-sink relations. Gene co-expression clusters containing CAM pathway genes are enriched with clock-associated cis-elements, suggesting circadian regulation of CAM. About 20% of pineapple microRNAs have diel expression patterns, with several that target key CAM-related genes. Expression and physiology data provide a model for CAM-specific carbohydrate flux and long-distance hexose transport. Together these resources provide a list of candidate genes for targeted engineering of CAM into C-3 photosynthesis crop species.
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