期刊
PLANT JOURNAL
卷 91, 期 4, 页码 590-600出版社
WILEY
DOI: 10.1111/tpj.13589
关键词
Arabidopsis thaliana; brassinosteroid receptor (BRI1); cGMP; kinase; guanylate cyclase; brassinosteroid signaling kinase 1 (BSK1)
资金
- Australian Research Council [DP0878194, DP110104164]
- National Research Foundation South Africa [78843, IRF2009021800047]
- La Trobe University School of Life Science Publication Booster Award
- Australian Research Council [DP0878194] Funding Source: Australian Research Council
The brassinosteroid receptor brassinosteroid insensitive 1 (BRI1) is a member of the leucine-rich repeat receptor-like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI1 generates pmol amounts of cGMP per mu g protein with a preference for magnesium over manganese as a co-factor. Importantly, a functional BRI1 kinase is essential for optimal cGMP generation. Therefore, the guanylate cyclase activity of BRI1 is modulated by the kinase while cGMP, the product of the guanylate cyclase, in turn inhibits BRI1 kinase activity. Furthermore, we show using Arabidopsis root cell cultures that cGMP rapidly potentiates phosphorylation of the downstream substrate brassinosteroid signaling kinase 1 (BSK1). Taken together, our results suggest that cGMP acts as a modulator that enhances downstream signaling while dampening signal generation from the receptor.
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