期刊
PLANT CELL AND ENVIRONMENT
卷 40, 期 9, 页码 1777-1790出版社
WILEY
DOI: 10.1111/pce.12982
关键词
excess ammonium; hydrogen sulfide; nitrogen assimilation; OsSE5; rice
资金
- National Natural Science Foundation of China [31670255]
- Fundamental Research Funds for the Central Universities [KYTZ201529, KYTZ201402]
- Natural Science Foundation of Jiangsu Province [BK20161447]
- State Key Laboratory of Crop Genetics & Germplasm Enhancement [ZW2013002]
- Royal Society (UK)
- Chinese Scholarship Council (CSC)
Previous studies revealed that rice heme oxygenase PHOTOPERIOD SENSITIVITY 5 (OsSE5) is involved in the regulation of tolerance to excess ammonium by enhancing antioxidant defence. In this study, the relationship between OsSE5 and hydrogen sulfide (H2S), a well-known signalling molecule, was investigated. Results showed that NH4Cl triggered the induction of L-cysteine desulfhydrase (L-DES)related H2S production in rice seedling roots. A H2S donor not only alleviated the excess ammonium-triggered inhibition of root growth but also reduced endogenous ammonium, both of which were aggravated by hypotaurine (HT, a H2S scavenger) or DL-propargylglycine (PAG, a L-DES inhibitor). Nitrogen metabolism-related enzymes were activated by H2S, thus resulting in the induction of amino acid synthesis and total nitrogen content. Interestingly, the activity of L-DES, as well as the enzymes involved in nitrogen metabolism, was significantly increased in the OsSE5-overexpression line (35S:OsSE5), whereas it impaired in the OsSE5-knockdown mutant (OsSE5-RNAi). The application of the HT/PAGorH2S donor could differentially block or rescue NH4Cl-hyposensitivity or hypersensitivity phenotypes in 35S:OsSE5-1 or OsSE5-RNAi-1 plants, with a concomitant modulation of nitrogen assimilation. Taken together, these results illustrated that H2S function as an indispensable positive regulator participated in OsSE5-promoted ammonium tolerance, in which nitrogen metabolism was facilitated.
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