期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 16, 期 1, 页码 50-62出版社
WILEY
DOI: 10.1111/pbi.12748
关键词
Haynaldia villosa L.; lectin receptor kinase; Triticum aestivum L.; powdery mildew resistance; transgenic wheat
资金
- National key research and development program [2016YFD0101004, 2016YFD0102001-004]
- National Science Foundation of China [31471490, 31661143005, 31290213]
- Important National Science & Technology Specific Projects in Transgenic Research [2014ZX0800907B]
- Chinese High Tech Program of China [2011AA1001]
- Program of Introducing Talents of Discipline to Universities [B08025]
- 333 Talent Project of Jiangsu Province
- Shanghai Agriculture Applied Technology Development Program, China Grant [Z201502]
Plant sense potential microbial pathogen using pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). The Lectin receptor-like kinase genes (LecRKs) are involved in various cellular processes mediated by signal transduction pathways. In the present study, an L-type lectin receptor kinase gene LecRK-V was cloned from Haynaldia villosa, a diploid wheat relative which is highly resistant to powdery mildew. The expression of LecRK-V was rapidly up-regulated by Bgt inoculation and chitin treatment. Its transcript level was higher in the leaves than in roots, culms, spikes and callus. Single-cell transient overexpression of LecRK-V led to decreased haustorium index in wheat variety Yangmai158, which is powdery mildew susceptible. Stable transformation LecRK-V into Yangmai158 significantly enhanced the powdery mildew resistance at both seedling and adult stages. At seedling stage, the transgenic line was highly resistance to 18 of the tested 23 Bgt isolates, hypersensitive responses (HR) were observed for 22 Bgt isolates, and more ROS at the Bgt infection sites was accumulated. These indicated that LecRK-V confers broad-spectrum resistance to powdery mildew, and ROS and SA pathways contribute to the enhanced powdery mildew resistance in wheat.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据