期刊
PLANT BIOSYSTEMS
卷 152, 期 5, 页码 1002-1010出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/11263504.2017.1407373
关键词
DGGE; real time PCR; nested qPCR; symbiosis; truffle mycobiome
The Tuber ascomata (truffles) are a microhabitat for bacteria, viruses, and fungi (yeasts and filamentous fungi). In this survey, we tried to develop a method that would make it possible to define the mycobiome of the truffle-inhabiting filamentous fungi using culture independent molecular methods. The nested quantitative Real-Time PCR allowed us to demonstrate that each truffle is home to multiple species of filamentous fungi and that their DNA is present within the healthy ascoma at the ratio of 10(-6) compared to that of the truffle. Probably due to their insignificant presence, Denaturing Gradient Gel Electrophoresis of the amplification of ITS amplicons showed only those of the host. Based on the results, the possibilities of being able to detect the fungicolous fungi present in very small amounts within a fungal host are discussed.
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