期刊
PLANT AND CELL PHYSIOLOGY
卷 58, 期 9, 页码 1594-1600出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcx084
关键词
Field-grown; Isoflavone; Rhizosphere; Soybean
资金
- Japan Society for the Promotion of Science (JSPS) [KAKENHI] [26712013, 26660279, 16K14901]
- Research Institute for Sustainable Humanosphere and Research Unit for Development of Global Sustainability, Kyoto University
- Ministry of Agriculture, Forestry and Fisheries of Japan [SFC2001]
- Grants-in-Aid for Scientific Research [26660279, 26712013, 16K14901] Funding Source: KAKEN
Isoflavones play important roles in rhizosphere plant-microbe interactions. Daidzein and genistein secreted by soybean roots induce the symbiotic interaction with rhizobia and may modulate rhizosphere interactions with microbes. Yet despite their important roles, little is known about the biosynthesis, secretion and fate of isoflavones in field-grown soybeans. Here, we analyzed isoflavone contents and the expression of isoflavone biosynthesis genes in field-grown soybeans. In roots, isoflavone contents and composition did not change with crop growth, but the expression of UGT4, an isoflavone-specific 7-O-glucosyltransferase, and of ICHG (isoflavone conjugates hydrolyzing beta-glucosidase) was decreased during the reproductive stages. Isoflavone contents were higher in rhizosphere soil than in bulk soil during both vegetative and reproductive stages, and were comparable in the rhizosphere soil between these two stages. We analyzed the degradation dynamics of daidzein and its glucosides to develop a model for predicting rhizosphere isoflavone contents from the amount of isoflavones secreted in hydroponic culture. Conjugates of daidzein were degraded much faster than daidzein, with degradation rate constants of 8.51 d(-1) for malonyldaidzin and 11.6 d(-1) for daidzin, vs. 9.15 x 10(-2) d(-1) for daidzein. The model suggested that secretion of isoflavones into the rhizosphere is higher during vegetative stages than during reproductive stages in field-grown soybean.
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