期刊
PLANT AND CELL PHYSIOLOGY
卷 58, 期 4, 页码 650-657出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcx033
关键词
Arabidopsis thaliana; Biochemistry; Chloroplast; Glutamine synthetase; Nitrogen metabolism
资金
- Precursory Research for Embryonic Science and Technology (PRESTO)
- Advanced Low Carbon Technology Research and Development Program (ALCA)
- Japan Society for the Promotion of Science [KAKENHI] [16H06559]
- Japan Science and Technology Agency [CREST]
- Grants-in-Aid for Scientific Research [16H06559] Funding Source: KAKEN
Glutamine synthetase (GS) is an important enzyme for nitrogen assimilation, and GS2, encoded by GLN2, is the only plastid-type GS in Arabidopsis thaliana. A co-expression analysis suggested that the expression level of the gene encoding a uridylyltransferase-like protein, ACR11, is strongly correlated with GLN2 expression levels. Here we showed that the recombinant ACR11 protein increased GS2 activity in vitro by reducing the Km values of its substrate glutamine. A T-DNA insertion mutant of ACR11 exhibited a reduced GS activity under low nitrate conditions and reduced glutamine levels. Biochemical analyses revealed that ACR11 and GS2 interacted both in vitro and in vivo. These data demonstrate that ACR11 is an activator of GS2, giving it a mechanistic role in the nitrogen assimilation of A. thaliana.
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