期刊
PIGMENT CELL & MELANOMA RESEARCH
卷 31, 期 3, 页码 374-383出版社
WILEY
DOI: 10.1111/pcmr.12668
关键词
D-tyrosine; L-tyrosine; melanin; melanogenesis; tyrosinase
资金
- Business for Cooperative R&D between Industry, Academy and Research Institute [C0443197]
- National Research Foundation of Korea (NRF) grant - Korea government (MSIP) [2017H1D2A2000669]
- National Research Foundation of Korea [2017H1D2A2000669] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Although L-tyrosine is well known for its melanogenic effect, the contribution of D-tyrosine to melanin synthesis was previously unexplored. Here, we reveal that, unlike L-tyrosine, D-tyrosine dose-dependently reduced the melanin contents of human MNT-1 melanoma cells and primary human melanocytes. In addition, 500 mu M of D-tyrosine completely inhibited 10 mu M L-tyrosine-induced melanogenesis, and both in vitro assays and L-DOPA staining MNT-1 cells showed that tyrosinase activity is reduced by D-tyrosine treatment. Thus, D-tyrosine appears to inhibit L-tyrosine-mediated melanogenesis by competitively inhibiting tyrosinase activity. Furthermore, we found that D-tyrosine inhibited melanogenesis induced by alpha-MSH treatment or UV irradiation, which are the most common environmental factors responsible for melanin synthesis. Finally, we confirmed that D-tyrosine reduced melanin synthesis in the epidermal basal layer of a 3D human skin model. Taken together, these data suggest that D-tyrosine negatively regulates melanin synthesis by inhibiting tyrosinase activity in melanocyte-derived cells.
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