期刊
ACS OMEGA
卷 3, 期 6, 页码 6976-6987出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.8b01086
关键词
-
资金
- U.K. BBSRC [BB/J001694/2]
- European Commission
- National Heart, Lung and Blood Institute, NIH, United States, through the NIH-Oxford Cambridge Research Scholars Program
- BBSRC [BB/J001694/2] Funding Source: UKRI
Dinucleoside phosphoramidites containing a triazole internucleotide linkage flanked by locked nucleic acid (LNA) were synthesized and incorporated into oligonucleotides (ONs). ONs bearing both LNA and triazole at multiple sites were obtained and their biophysical properties including enzymatic stability and binding affinity for RNA and DNA targets were studied. t-LNAs with four incorporations of a dinucleoside monomer having LNA on either side of the triazole linkage bind to their RNA target with significantly higher affinity and greater specificity than unmodified oligonucleotides, and are remarkably stable to nuclease degradation. A similar but reduced effect on enzymatic stability and binding affinity was noted for LNA only on the 3'-side of the triazole linkage. Thus, by combining unnatural triazole linkages and LNA in one unit (t-LNA), we produced a promising class of ONs with reduced anionic charge and potential for antisense applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据