期刊
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
卷 3, 期 2, 页码 97-104出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.synbio.2018.02.004
关键词
Absolute quantification; Mass spectrometry; Cell-free protein synthesis system; In vitro translation; Targeted quantitative proteomics; PURE system
资金
- Japan Society for the Promotion of Science (JSPS) [17H05680]
- strategic programs for R&D (President's discretionary fund) of RIKEN
- RIKEN Quantitative Biology Center
- Grants-in-Aid for Scientific Research [17H05680] Funding Source: KAKEN
High-sensitivity mass spectrometry approaches using selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) methods are powerful tools for targeted quantitative proteomics-based investigation of dynamics in specific biological systems. Both high-sensitivity detection of low-abundance proteins and their quantification using this technique employ stable isotope-labeled peptide internal standards. Currently, there are various ways for preparing standards, including chemical peptide synthesis, cellular protein expression, and cell-free protein or peptide synthesis. Cell-free protein synthesis (CFPS) or in vitro translation (IVT) systems in particular provide high-throughput and low-cost preparation methods, and various cell types and reconstituted forms are now commercially available. Herein, we review the use of such systems for precise and reliable protein quantification. (c) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co.
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