期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 136, 期 -, 页码 41-45出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2016.08.006
关键词
Flixweed; ALS; Cross-resistance; Trp-574-Leu mutation
资金
- China Special Fund for Agro-scientific Research in the Public Interest [201303031]
Acetolactate synthase (ALS) is the common target of ALS-inhibiting herbicides, and target-site ALS mutations are the main mechanism of resistance to ALS-inhibiting herbicides. In this study, ALS1 and ALS2 genes with full lengths of 2004 bp and 1998 bp respectively were cloned in individual plants of susceptible (S) or resistant (R) flixweed (Descurainia sophia L) populations. Two ALS mutations of Pro-197-Thr and/or Trp-574-Leu were iden-tified in plants of three R biotypes (HB24, HB30 and HB42). In order to investigate the function of ALS isozymes in ALS-inhibiting herbicide resistance, pHB24 (a Pro-197-Thr mutation in ALS1 and a wild type ALS2), pHB42 (a Trp-574-Leu mutation in ALS1 and a wild type ALS2) and pHB30 (a Trp-574-Leu mutation in ALS1 and a Pro-197-Thr mutation in ALS2) subpopulations individually homozygous for different ALS mutations were gen-erated. Individuals of pHB30 had mutations in each isozyme of ALS and had higher resistance than pHB24 and pHB42 populations containing mutations in only one ALS isozyme. Moreover, the pHB24 had resistance to SU, TP and SCT herbicides, whereas pHB24 and pHB42 had resistance to these classes of herbicides as well as IMI and PTB herbicides. The sensitivity of isolated ALS enzyme to inhibition by herbicides in these populations corre-lated with whole plant resistance levels. Therefore, reduced ALS sensitivity resulting from the mutations in ALS was responsible for resistance to ALS-inhibiting herbicides in flixweed. (C) 2016 Elsevier Inc. All rights reserved.
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