期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 141, 期 -, 页码 71-75出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2016.11.011
关键词
Biological control; Fungicide-tolerance; Chemical induction; Nematophagous fungi
资金
- Special Fund for Agri-scientific Research in the Public Interest of China [201503114]
- Special Project of the National Key Research and Development Program [2017YFD]
- National Key Technology Support Program [2012BAD15B03]
- Agricultural Science and Technology Innovation Program
- Knowledge Innovation Program of the Chinese Academy of Sciences (CASKIP)
- Chinese Academy of Agricultural Sciences [SFBW-CAAS-2017]
Lecanicillium attenuatum is an important nematophagous fungus with potential as a biopesticide for control of plant-pathogenic nematodes. However, relatively low fungicide-tolerance limits its application in the field. To improve the propamocarb-tolerance of L. attenuatum, a NTG-based mutagenesis system was established. Among different combinations of NTG concentration and treatment time in the first-round NTG treatment, the treatment of 1.0 mg/ml NTG for 60 min gave a proper conidial lethality rate of 84.6% and the highest positive mutation rate of 7.7%, and then produced the highest propamocarb-tolerant mutant LA-C-R1-T4-M whose EC50 value reached to 1050.0 mu g/ml. The positive mutation range was 105.1% in the first-round NTG treatment. Multiple -round NTG treatment was further employed to enhance the propamocarb tolerance of L attenuatum. The positive mutation range was significantly accumulated to 179.3% on the third-round NTG treatment, and then appeared to level-off and remained constant. These results indicated that multiple-round NTG treatment had a significant accumulative effect on fungal tolerance to propamocarb. Among all chemical-mutants, the LA-C-R3-M was the highest tolerant to propamocarb, whose EC50 value was increased 2.79-fold compared to the wild-type strain, and it was mitotic stable after 20 passages on PDA medium. Colony growth, conidia yield and conidial germination on plates, and parasitism of nematode eggs of M. incognita and H. glycines were not significantly changed by the NTG-based mutagenesis compared to the wild-type strain in either single- or multiple-round NTG treatment. In conclusion, we succeeded in improving the propamocarb tolerance of L. attenuatum via the optimized NTG-based mutagenesis system. The improved strain LA-C-R3-M could be potentially applied with propamocarb in the field. (C) 2016 Elsevier Inc. All rights reserved.
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