期刊
PEST MANAGEMENT SCIENCE
卷 74, 期 3, 页码 569-578出版社
WILEY
DOI: 10.1002/ps.4738
关键词
Bactrocera dorsalis; tyrosine hydroxylase; larval-pupal; tanning and immunity; RNA interference; 3-iodo-tyrosine
资金
- National Key Research and Development Program [2016YFC1200600]
- National Natural Sciences Foundation [31572016, 31301667]
- Fundamental Research Funds for the Central Universities, China [2362015xk04]
- earmarked fund for Modern Agro-industry (Citrus) Technology Research System of China [CARS-26]
- Foundation Project of Southwest University [SWU114049]
BACKGROUND: The oriental fruit fly Bactrocera dorsalis (Hendel), a notorious world pest infesting fruits and vegetables, has evolved a high level of resistance to many commonly used insecticides. In this study, we investigate whether tyrosine hydroxylase (TH) that is required for cuticle tanning (sclerotization and pigmentation) in many insects, could be a potential target in controlling B. dorsalis. RESULTS: We cloned TH cDNA (BdTH) of B. dorsalis. The complete open reading frame of BdTH (KY911196) was 1737 bp in length, encoding a protein of 578 amino acids. Quantitative real-time PCR confirmed that BdTH was highly expressed in the epidermis of 3rd instar larvae, and its expression increased prior to pupation, suggesting a role in larval-pupal cuticle tanning. When we injected dsBdTH or 3-iodo-tyrosine (3-IT) as a TH inhibitor or fed insect diet supplemented with 3-IT, there was significant impairment of larval-pupal cuticle tanning and a severe obstacle to eclosion in adults followed by death in most. Furthermore, injection of Escherichia coli into larvae fed 3-IT resulted in 92% mortality and the expressions of four antimicrobial peptide genes were significantly downregulated. CONCLUSION: These results suggest that BdTH might play a critical role in larval-pupal tanning and immunity of B. dorsalis, and could be used as a potential novel target for pest control. (C) 2017 Society of Chemical Industry
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