期刊
PEPTIDES
卷 98, 期 -, 页码 15-22出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2016.12.017
关键词
Insect; Neuropeptide; Satiety; Hindgut contraction; Immunohistochemistry; In situ hybridizationa
资金
- NSERC Discovery grants
Sulfakinins (SKs) are a family of multifunctional neuropeptides that have been shown to have myotropic activity on muscles of the digestive system and to function as feeding satiety factors. Here, we confirm via cloning the presence of two sulfakinins (Rhopr-SK-1 and Rhopr-SK-2) in Rhodnius prolixus. Reverse transcriptase quantitative PCR demonstrates that the Rhopr-SK transcript is highly expressed in the central nervous system (CNS) of unfed fifth-instar R. prolixus. Fluorescent in situ hybridization shows transcript expression only in neurons in the brain. Immunohistochemical staining of SK-like peptides was observed in the same neurons in the brain and in processes extending throughout the CNS, as well as over the posterior midgut and anterior hindgut. Rhopr-SK-1 (sulfated form) induces contractions of the hindgutin a dose-dependent manner. Injection Rhopr-SK-1 (sulfated form) significantly decreases the overall weight of the blood meal consumed, suggesting SK's role as a satiety factor in R. prolixus. (C) 2016 Elsevier Inc. All rights reserved.
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