期刊
PEPTIDES
卷 95, 期 -, 页码 84-93出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2017.07.012
关键词
Algesia; Edema; Hemolysis; Multiple alanine residues; Peptide; Wasp venom
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [444292/2014-4, 490447/2013-9, 407625/2013-5]
- Consejo Nacional de Ciencia y Tecnologia - CONACyT [209918]
- Fundacao de Empreendimentos Cientificos e Tecnologicos - FINATEC
- Fundacao de Apoio a Pesquisa do Distrito Federal - FAPDF [0193.000494/2015]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES
Chatergellus communis is a wasp species endemic to the neotropical region. and its venom constituents have never been described. In this study, two peptides from C. communis venom, denominated Communis and Communis-AAAA, were chemically and biologically characterized. In respect to the chemical characterization, the following amino acid sequences and molecular masses were identified: Communis: Ile-Asn-Trp-Lys-Ala-Ile-Leu-Gly-Lys-Ile-Gly-Lys-COOH (1340.9 Da) Communis-AAAA: Ile-Asn-Trp-Lys-Ala-Ile-Leu-Gly-Lys-Ile-Gly-Lys-Ala-Ala-Ala-Ala-Val-Xle-NH2 (1836.3 Da). Furthermore, their biological effects were compared, accounting for the differences in structural characteristics between the two peptides. To this end, three biological assays were performed in order to evaluate the hyperalgesic, edematogenic and hemolytic effects of these molecules. Communis-AAAA, unlike Communis, showed a potent hemolytic activity with EC50 = 142.6 mu M. Moreover, the highest dose of Communis-AAAA (2nmol/animal) induced hyperalgesia in mice. On the other hand, Communis (10nmol/animal) was able to induce edema but did not present hemolytic or hyperalgesic activity. Although both peptides have similarities in linear structures, we demonstrated the distinct biological effects of Communis and Commtmis-AAAA. This is the first study with Chartegellus communis venom, and both Communis and Communis-AAAA are unpublished peptides.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据