期刊
FEMS MICROBIOLOGY ECOLOGY
卷 94, 期 10, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiy143
关键词
Staphylococcus epidermidis; bacteriophage; flow cytometry; stationary cells; gene expression; phage-host interactions
类别
资金
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469]
- BioTecNorte operation - European Regional Development Fund [NORTE-01-0145-FEDER-000004]
- FCT fellowship [SFRH/BPD/99961/2014]
- COMPETE 2020 [POCI-01-0145-FEDER-006684]
The majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells suggests that SEP1 infection leads to the upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells.
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