期刊
ACS OMEGA
卷 3, 期 10, 页码 12886-12892出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.8b01812
关键词
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资金
- National Natural Science Foundation of China [51573068, 21674041]
- Program for Changbaishan Scholars of Jilin Province of China
A facile, sensitive, and label-free aptamer-based fluorescent biosensor (aptasensor) for chloramphenicol (CAP) detection was successfully developed based on an aggregation-induced emission (AIE) probe and graphene oxide (GO). In this aptasensor, the specific aptamer of CAP (C-Apt) is used as the recognition part, an AIE molecule, 9,10-distyrylanthracene (DSA) derivative with short alkyl chains (9,10-bis{4-[2-(N,N,N-trimethylammonium)-ethoxy]-styrene}anthracene dbromide, DSAC(2)N), as the fluorescent probe; and GO with a low oxidation degree as the fluorescent quencher. Initially, the AIE probe DSAC(2)N and C-Apt could be adsorbed on GO through pi-stacking interactions, and the fluorescence of DSAC(2)N could be efficiently quenched due to the energy transfer between DSAC(2)N and GO. When CAP is added, C-Apt can preferentially bind with CAP and the newly formed complex (C-Apt-CAP) can be released from GO, resulting in the recovery of the fluorescence signal of DSAC(2)N. Thus, with the aid of GO, turn-on detection of CAP can be readily realized by monitoring the fluorescence signal of DSAC(2)N from off to on. Under the optimized conditions, the aptasensor exhibits a high sensitivity toward CAP with a limit of detection of 1.26 pg/mL. Besides, we have successfully applied this aptasensor to the detection of CAP in spiked milk.
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