期刊
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
卷 2017, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2017/8940321
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类别
资金
- Fundacao para a Ciencia e Tecnologia (FCT) [PTDC/SAU-MIC/111447/2009, SFRH/BPD/79224/2011, PD/BD/113990/2015]
- Ministero dell'Istruzione, dell'Universita e della Ricerca of Italy [PRIN 20158EB2CM-003]
- Consiglio Nazionale delle Ricerche of Italy
- FCT
- Federation of European Biochemical Societies
- Fundacao para a Ciencia e Tecnologia/Ministerio da Ciencia e do Ensino Superior, through national funds [LISBOA-01-0145FEDER-007344]
- FEDER [PT2020]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/79224/2011, PTDC/SAU-MIC/111447/2009, PD/BD/113990/2015] Funding Source: FCT
The human disease classical homocystinuria results from mutations in the gene encoding the pyridoxal 5'-phosphate- (PLP-) dependent cystathionine beta-synthase (CBS), a key enzyme in the transsulfuration pathway that controls homocysteine levels, and is a major source of the signaling molecule hydrogen sulfide (H2S). CBS activity, contributing to cellular redox homeostasis, is positively regulated by s-adenosyl-l-methionine (AdoMet) but fully inhibited upon CO or NO. binding to a noncatalytic heme moiety. Despite extensive studies, the molecular basis of several pathogenic CBS mutations is not yet fully understood. Here we found that the ferrous heme of the reportedly mild p.P49L CBS variant has altered spectral properties and markedly increased affinity for CO, making the protein much more prone than wild type(WT) CBS to inactivation at physiological CO levels. The higher CO affinity could result from the slightly higher flexibility in the heme surroundings revealed by solving at 2.80-angstrom A resolution the crystallographic structure of a truncated p.P49L. Additionally, we report that p.P49L displays impaired H2S-generating activity, fully rescued by PLP supplementation along the purification, despite a minor responsiveness to AdoMet. Altogether, the results highlight how increased propensity to CO inactivation of an otherwise WT-like variant may represent a novel pathogenic mechanism in classical homocystinuria.
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