期刊
ACS OMEGA
卷 3, 期 8, 页码 9630-9635出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.8b01551
关键词
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资金
- National Cancer Institute [R01 CA090689]
- NCI Cancer Center Support grant [P30 CA042014]
We have studied the in vitro stability of 25 potential i-motif-forming DNA sequences found within the promoter regions of 18 different human DNA repair genes. Three widely available methods of characterization were used to rapidly assess i-motif folding and stability and comprise a simple screen for preliminary identification of physiologically relevant i-motif forming sequences. Four highly pH-stable candidate sequences were identified exhibiting pH transitions (pH at which 50% of the oligodeoxynucleotides in solution are folded) at or above pH 6.6, thermal melting temperatures above 37 degrees C and isothermal UV difference spectra characteristic of 2'-deoxycytidine imino-nitrogen protonation. These newly identified i-motif forming sequences could represent novel targets for understanding and modulating human DNA repair gene expression.
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