期刊
OSTEOARTHRITIS AND CARTILAGE
卷 25, 期 9, 页码 1551-1562出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2017.05.007
关键词
Temporomandibular joint; Osteoarthritis; Cartilage; CD163; Bone marrow mesenchymal stem cells
资金
- National Natural Science Foundation of China [81530033, 81630066, 81500875, 81500896, 81472049]
- Health Science Foundation of Shanxi Province [2016D009]
- Heilongjiang Young Investigator Natural Science Foundation [QC2016115]
Objectives: The present goal was to explore whether matrix replenishment is the primary requirement for osteoarthritic (OA) cartilage. Methods: Cells isolated from the superficial and deep zone cartilage of a pig temporomandibular joint (TMJ) were exposed to fluid flow shear stress (FFSS). Differences in matrix production and cellular differentiation were detected. Unilateral anterior crossbite (UAC) was applied to C57BL/6J female mice. Green fluorescent protein-labeled exogenous bone marrow stromal cells (GFP-BMSCs) were injected weekly into TMJs, starting from 3 weeks of UAC stimulation and continuing for 4-, 8- and 12-weeks. Another GFP-BMSCs injection UAC group stopped receiving injections for 4-weeks after 8-weeks of injections. Assessments were focused on morphological alterations in UAC mouse TMJ cartilage, the expression levels of DAP3, an anoikis marker, CD163, a scavenger receptor family member, and ki67, a proliferation indicator. Results: FFSS down-regulated type-II collagen expression but stimulated terminal differentiation in cells isolated from deep zone cartilage. It down-regulated aggrecan expression but up-regulated type I collagen in cells isolated from both superficial and deep zones. UAC caused matrix loss and anoikis and enhanced scavenging activity in deep zone chondrocytes without affecting cell proliferation. Superficial fibrillation was obvious in the late stage. Weekly injections of BMSCs largely restored these changes. The implanted BMSCs expressed a high level of CD163 protein but did not show remarkable cell proliferation. Terminating the supply of exogenous BMSCs reversed the restorative effects. Conclusions: Scavenging the degraded matrix and replenishing the fibrosis-developmental matrix are the primary requirements for the repair of OA cartilage. (C) 2017 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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