期刊
ANALYTICAL CHEMISTRY
卷 90, 期 20, 页码 11756-11759出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b03268
关键词
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资金
- NIH [R33 CA225248, R21 EB020976]
- Pacific Northwest Prostate Cancer Grant [SPORE/NCI P50 CA097186]
- OHSU-Knight Cancer Institute
- Earth & Biological Sciences Directorate Mission Seed under the Laboratory Directed Research and Development Program at PNNL
- Precision Medicine Innovation Co-Laboratory (PMedIC)
- Department of Energy's Office of Biological and Environmental Research
Proteome profiling of circulating tumor cells (CTCs) can provide crucial insight into disease progression and the role of CTCs in tumor metastasis. We describe an integrated workflow to measure global protein expression in 1-5 spiked CTCs enriched from whole blood by immunodensity gradient centrifugation. Enriched CTCs were purified and collected by laser capture microdissection, prepared using a recently developed nanodroplet-based processing platform (nanoPOTS), and finally analyzed by ultrasensitive nanoLC-MS/MS. The workflow was capable of identifying an average of 164 and 607 protein groups from samples comprising 1 and 5 LNCaP cells, respectively, that were isolated from human whole blood. A panel of prostate cancer-specific proteins were identified and quantified, which was used to differentiate between spiked CTCs and white blood cells.
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