期刊
GENOME BIOLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13059-018-1547-5
关键词
-
资金
- Northeastern University
- NIGMS from the National Institutes of Health [DP2GM123497]
Some exciting biological questions require quantifying thousands of proteins in single cells. To achieve this goal, we develop Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) and validate its ability to identify distinct human cancer cell types based on their proteomes. We use SCoPE-MS to quantify over a thousand proteins in differentiating mouse embryonic stem cells. The single-cell proteomes enable us to deconstruct cell populations and infer protein abundance relationships. Comparison between single-cell proteomes and transcriptomes indicates coordinated mRNA and protein covariation, yet many genes exhibit functionally concerted and distinct regulatory patterns at the mRNA and the protein level.
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