期刊
COGENT CHEMISTRY
卷 4, 期 1, 页码 1-10出版社
TAYLOR & FRANCIS AS
DOI: 10.1080/23312009.2018.1529259
关键词
Kaffir lime; 6 ',7 '-dihydroxybergamottin; oxypeucedanin hydrate; inflammatory mediators
资金
- Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University
- Cluster of Excellence on Biodiversity-based Economy and Society (B. BES-CMU), Chiang Mai University
Two known furanocoumarins, namely, 6',7'-dihydroxybergamottin (1) and oxypeucedanin hydrate (2), were isolated from Kaffir lime (Citrus hystrix DC) fruit peel using an assay of nitric oxide scavenging effect-guided fractionation. The inhibitory activities of isolated coumarins on the production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) were studied in mouse macrophages RAW264.7 cells, whereas their inhibitory effect on cyclooxygenase-2 (COX-2) production was investigated using HT-29 and HCT116 cells. The results demonstrated that 1 inhibited lipopolysaccharide-interferon gamma-induced NO and iNOS production in RAW264.7 cells and COX-2 production in HT-29 and HCT116 cells with IC50 values of 16.16 +/- 1.08, 18.63 +/- 1.42, 18.19 +/- 0.95 and 17.53 +/- 0.88 mu g/mL, respectively; 2 showed IC50 values of 18.23 +/- 1.25, 22.54 +/- 1.56, 22.27 +/- 1.14 and 23.24 +/- 1.05 mu g/mL, respectively. Additionally, the positive control curcumin exhibited these activities with IC50 values of 12.52 +/- 0.63, 15.55 +/- 1.34, 14.29 +/- 0.58 and 16.14 +/- 0.65 mu g/mL, respectively. The inhibitory effect of 1 on the production of iNOS and COX-2 was significantly stronger than that of 2. This study indicated that furanocoumarins from Kaffir lime fruit peels have potency for further development as an anti-inflammatory drug.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据