期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 15, 期 14, 页码 2947-2952出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ob00220c
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资金
- BBSRC
- BRC
- HEFCE
- EPSRC
- BBSRC [BB/L024349/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L024349/1] Funding Source: researchfish
Disulfide bridging offers a convenient approach to generate site-selective antibody conjugates from native antibodies. To optimise the reagents available to achieve this strategy, we describe here the use of dibromomaleimides designed to undergo accelerated post-conjugation hydrolysis. Conjugation and hydrolysis, which serve to 'lock' the conjugates as robustly stable maleamic acids, is achieved in just over 1 h. This dramatic acceleration is also shown to infer significant improvements in homogeneity, as demonstrated by mass spectrometry analysis.
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