期刊
MICRO AND NANO ENGINEERING
卷 1, 期 -, 页码 25-32出版社
ELSEVIER
DOI: 10.1016/j.mne.2018.10.003
关键词
Cell-free circulating DNA; Body fluid nucleic acid biomarkers; DNA separation; DNA concentration
类别
资金
- LAAS-CNRS micro and nanotechnologies platform, member of the French RENATECH network
- Institut Carnot
- Fondation pour la Recherche Medicale
- project Gene Extractor from RegionMidi-Pyrenees
- ANR project muLAS [ANR-16-CE18-0028-01]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE18-0028] Funding Source: Agence Nationale de la Recherche (ANR)
Genetic testing based on the analysis of circulating cell-free DNA (cfDNA) in body fluids, especially blood plasma, is raising interest for the management and follow-up of many diseases, including cancer. Because the concentration of cfDNA is low and its composition mostly degraded, this material can only be assayed with the most sensitive nucleic acid processing technologies. cfDNA analysis therefore constitutes a model target and a driving force for innovation in microfluidic biotechnologies. Here, we overview the main physico-chemical characteristics of cfDNA, and provide a critical review on the different methods for its processing out of blood samples. Then, we describe recent microfluidic developments for high sensitivity DNA analysis, evaluate their practical relevance for cfDNA analysis, and identify a few challenges for technologists in the near future. (c) 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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