期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 50, 期 10, 页码 1028-1037出版社
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gmy101
关键词
posterior capsule opacification (PCO); HOX transcript antisense RNA (HOTAIR); epithelial-mesenchymal transition (EMT); transforming growth factor beta 2 (TGF-beta 2)
资金
- National Natural Science Foundation of China [81570841]
- Sichuan Provincial Department of Health [12062]
Posterior capsule opacification (PCO) results from the proliferation, migration, and epithelial-mesenchymal transition (EMT) of residual lens epithelial cells (LECs) and fibers in the capsular bag. Previous reports have demonstrated that transforming growth factor beta 2 (TGF-beta 2) affects the cellular processes via modulation of EMT in LECs. However, the mechanisms that underlie the TGF-beta 2-induced EMT in LECs are still largely unknown. In this study, we confirmed that TGF-beta 2 induces EMT in SRA01/04 cells via the up-regulation of the long non-coding RNA (lncRNA) HOTAIR. To study the effects of HOTAIR on the proliferation, migration and EMT of SRA01/04 cells as well as the underlying mechanism, we used small interfering RNA (siRNA) to specifically attenuate HOTAIR expression in SRA01/04 cells. CCK8 cell-counting kit was used to examine SRA01/04 cell viability; EdU cell proliferation kit was used to examine SRA01/04 cell proliferation; Transwell system and scratch assays were used to observe cell migration; and qPCR and western blot analysis were used to evaluate EMT progression. We found that inhibition of HOTAIR expression repressed SRA01/04 cell viability, proliferation, migration and prevented the TGF-beta 2-induced changes in cellular processes via modulation of EMT. Ultimately, we found that HOTAIR affected the TGF-beta/Smad signaling pathway. In summary, we elucidated that HOTAIR affected the cell viability, proliferation, and migration in the TGF-beta 2-induced EMT in SRA01/04 cells and suggested that modulation of HOTAIR may be helpful in PCO prevention and therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据