Determination of the presence of transgenic soybean in Indonesian commercial soybean and tempeh using 35S promoter primer by real-time polymerase chain reaction

The present of genetically modified organism (GMO) in food materials such as soybeans regulated by Indonesian Food and Drug Administration. To impose the law, reliable methods to detect transgenic component are needed. Validation of the real-time polymerase chain reaction (real time-PCR) to detect the presence of transgenic in soybeans and tempeh has been accomplished. The method used a specific primer to amplify promoter 35S. The method could correctly distinguish GMO soybeans from non-transgenic one. The precision of the method is high as represented by CV value of Ct, which is 7.1% (acceptance criteria < 25%). The real-time PCR still give precise fluorescent signal up to 5pg of DNA template, as a representation of limit of detection of the methods. By the method, one commercial soybean labeled as non-GMO has been detected as GMO soybean, while most imported soybeans as well as the commercial tempeh are GMO. (C) All Rights Reserved