期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 50, 期 2, 页码 552-568出版社
KARGER
DOI: 10.1159/000494168
关键词
Dexmedetomidine; Circulating miRNAs; Expression profiling; Ischemia; reperfusion injury; Cardioprotection
资金
- National Natural Science Foundation of China [81460044, 81860050, 81160035]
- Yunnan Provincial Science and Technology Department [2014HB031, 2017FE468 (-039)]
- Health and Family Planning Commission of Yunnan Province [2018NS0126]
- Program for Innovative Research Team of Kunming Medical University [CXTD201802]
- Yunnan health training project of high level talents
Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine. (c) 2018 The Author(s) Published by S. Karger AG, Basel
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