期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/ijms19102990
关键词
osteopontin; papillary thyroid carcinoma; psammoma bodies; matrix calcification; OPNa
资金
- CNPq [237322/2012-9]
- FCT [SFRH/BD/87887/2012, SFRH/BD/110617/2015]
- FEDER Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020
- Operacional Programme for Competitiveness and Internationalization POCI [POCI-01-0145-FEDER-007274]
- Projetos Estruturados de I & D & I - Norte 2020-Programa Operacional Regional do Norte
- [NORTE-01-0145-FEDER-000029]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/110617/2015, SFRH/BD/87887/2012] Funding Source: FCT
Osteopontin (OPN) spliced variants (OPN-SV: OPNa, OPNb, and OPNc) are aberrantly expressed in tumors and frequently associated with cancer progression. This holds true for papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer (TC). PTC often presents with desmoplasia and dystrophic calcification, including psammoma bodies (PB). This work aimed to investigate total OPN (tOPN) and OPN-SV expression and their association with the presence of PB in the PTC classical variants (cPTC), as well as the involvement of OPN-SV in matrix calcification of TC cell lines. We found that cPTC samples presenting PB showed higher OPN expression levels. In TC cell lines, OPNa overexpression promotes higher matrix calcification and collagen synthesis when compared to that of clones overexpressing OPNb or OPNc. In response to OPN knockdown, calcification was inhibited, paralleled with the downregulation of calcification markers. In conclusion, our data evidenced that OPN expression is associated with the presence of PB in cPTC samples. Among the OPN-SV, OPNa is the main contributor to matrix calcification in tested TC cells, providing clues to a better understanding on the biology and ethiopathogenesis of the calcification process in TC cells.
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