期刊
ONCOLOGY RESEARCH
卷 25, 期 8, 页码 1317-1327出版社
TECH SCIENCE PRESS
DOI: 10.3727/096504017X14874323871217
关键词
Glioma; Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5); Proliferation; Migration; Invasion
类别
资金
- National Natural Science Foundation of China [81301041, 31371501, 81400382]
- Scientific Research Program - Department of Science and Technology of Shaanxi Province [2015KJXX-43]
- Leading Disciplines Development Government Foundation of Shaanxi, China [(2014)3-1001]
- Xi'an Medical University [2016PT06]
Increasing studies have suggested that microRNAs (miRNAs) are involved in the development of gliomas. MicroRNA-216a has been reported to be a tumor-associated miRNA in many types of cancer, either as an oncogene or as a tumor suppressor. However, little is known about the function of miR-216a in gliomas. The present study was designed to explore the potential role of miR-216a in gliomas. We found that miR-216a was significantly decreased in glioma tissues and cell lines. Overexpression of miR-216a significantly suppressed the proliferation, migration, and invasion of glioma cells. Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) was identified as a target gene of miR-216a in glioma cells by bioinformatics analysis, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis. Moreover, miR-216a overexpression inhibited the Wnt/beta-catenin signaling pathway. The restoration of LGR5 expression markedly reversed the antitumor effect of miR-216a in glioma cells. Taken together, these findings suggest a tumor suppressor role for miR-216a in gliomas, which inhibits glioma cell proliferation, migration, and invasion by targeting LGR5. Our study suggests that miR-216a may serve as a potential therapeutic target for future glioma treatment.
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