期刊
ONCOLOGY REPORTS
卷 37, 期 6, 页码 3287-3296出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2017.5590
关键词
autophagy; AMPK pathway; NSCLC cells; Akt/mTOR pathway
类别
资金
- Creative Fusion Research Program through Creative Allied Project - Korean Research Council for Fundamental Science and Technology [CAP-12-1-KIST]
- Korea Basic Science Institute Research Program [C36955]
- Korea Institute of Oriental Medicine [K16060]
- National Research Council of Science & Technology (NST), Republic of Korea [CAP-12-1-KIST, K16060, CAP-12-1--KIST] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Panax ginseng has been used worldwide as a traditional medicine for the treatment of cancer and other diseases. The antiproliferative activity of ginseng has been increased after enzymatic processing of ginseng saponin, which may result in the accumulation of minor saponins, such as Rh2, Rg3, compound K and protopanaxatriol type (PPT) in modified regular ginseng extract (MRGX). In the present study, the anticancer activity and the associated mechanisms of MRGX were investigated using A549 human lung cancer cells. To elucidate the mechanisms underlying the effects of MRGX, we performed a microarray analysis of gene expression in the A549 cells. Molecular mechanisms that were associated with the anticancer activity of MRGX were studied, with a special focus on the autophagy-related multiple signaling pathways in lung cancer cells. Microarray analyses elucidated autophagy-related genes affected by MRGX. Administration of MRGX at 100 mu g/ml induced punctate cytoplasmic expression of LC3, Beclin-1 and ATG5 and increased expression of endogenous LC3-II whereas 50 mu g/ml did not inhibit the proliferation of A549 cells. Compared to the control cells, in cells treated with MRGX at 100 mu g/ml, the level of p-Akt was increased, while that of mTOR-4EBP1 was decreased. Downregulation of mTOR and 4EBP1 in the MRGX-treated cells was found not to be a p-Ulk (S757)-dependent pathway, but a p-Ulk (S317)-dependent autophagic pathway, using AMPK. These data suggest that MRGX regulates AMPK and induces autophagy in lung cancer cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据