期刊
NUCLEIC ACIDS RESEARCH
卷 45, 期 12, 页码 7487-7493出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx274
关键词
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资金
- Singapore Ministry of Education [MOE2015-T2-1-092]
- National Research Foundation [NRF-NRFI2017-09]
- Nanyang Technological University
AGRO100 (also known as AS1411) is a G-rich oligonucleotide that has long been established as a potent anti-cancer aptamer. However, the structure of AGRO100 remained unresolved, due to the co-existence of multiple different G-quadruplex conformations. We identified a DNA sequence named AT11, derived from AGRO100, which formed a single major G-quadruplex conformation and exhibited a similar anti-proliferative activity as AGRO100. The solution structure of AT11 revealed a four-layer G-quadruplex comprising of two propeller-type parallel-stranded subunits connected through a central linker. The stacking between the two subunits occurs at the 3'-end of the first block and the 5'-end of the second block. The structure of the anti-proliferative DNA sequence AT11 will allow greater understanding on the G-quadruplex folding principles and aid in structural optimization of anti-proliferative oligonucleotides.
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