期刊
NUCLEIC ACIDS RESEARCH
卷 45, 期 21, 页码 12152-12169出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx798
关键词
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资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI Grants [JP16H04720, JP16H01216, JP25.5081]
- Japan Science and Technology Agency-Exploratory Research for Advanced Technology (JST-ERATO) Grant [JPMJER1104]
- JSPS KAKENHI Grant [JP16H04720]
- Grants-in-Aid for Scientific Research [16H01216, 16H04720] Funding Source: KAKEN
Single transcription factors (TFs) regulate multiple developmental pathways, but the underlying mechanisms remain unclear. Here, we quantitatively characterized the genome-wide occupancy profiles of BLIMP1, a key transcriptional regulator for diverse developmental processes, during the development of three germ-layer derivatives (photoreceptor precursors, embryonic intestinal epithelium and plasmablasts) and the germ cell lineage (primordial germ cells). We identified BLIMP1-binding sites shared among multiple developmental processes, and such sites were highly occupied by BLIMP1 with a stringent recognition motif and were located predominantly in promoter proximities. A subset of bindings common to all the lineages exhibited a new, strong recognition sequence, a GGGAAA repeat. Paradoxically, however, the shared/common bindings had only a slight impact on the associated gene expression. In contrast, BLIMP1 occupied more distal sites in a cell type-specific manner; despite lower occupancy and flexible sequence recognitions, such bindings contributed effectively to the repression of the associated genes. Recognition motifs of other key TFs in BLIMP1-binding sites had little impact on the expression-level changes. These findings suggest that the shared/common sites might serve as potential reservoirs of BLIMP1 that functions at the specific sites, providing the foundation for a unified understanding of the genome regulation by BLIMP1, and, possibly, TFs in general.
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