期刊
NUCLEIC ACIDS RESEARCH
卷 45, 期 18, 页码 10481-10491出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx676
关键词
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资金
- National Institutes of Health (NIH) [R01GM047477]
- NIH [GM047477]
Ribosomal protein (RP) genes must be coordinately expressed for proper assembly of the ribosome yet themechanisms that control expression of RP genes in metazoans are poorly understood. Recently, TATAbinding protein-related factor 2 (TRF2) rather than the TATA-binding protein (TBP) was found to function in transcription of RP genes in Drosophila. Unlike TBP, TRF2 lacks sequence-specific DNA binding activity, so the mechanism by which TRF2 is recruited to promoters is unclear. We show that the transcription factor M1BP, which associates with the core promoter region, activates transcription of RP genes. Moreover, M1BP directly interacts with TRF2 to recruit it to the RP gene promoter. High resolution ChIP-exo was used to analyze in vivo the association of M1BP, TRF2 and TFIID subunit, TAF1. Despite recent work suggesting that TFIID does not associate with RP genes in Drosophila, we find that TAF1 is present at RP gene promoters and that its interactionmight also be directed by M1BP. Although M1BP associates with thousands of genes, its colocalization with TRF2 is largely restricted to RP genes, suggesting that this combination is key to coordinately regulating transcription of the majority of RP genes in Drosophila.
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