期刊
COMMUNICATIONS PHYSICS
卷 1, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s42005-018-0092-6
关键词
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资金
- Swedish Research Council
- Knut and Alice Wallenberg Foundation
- European Research Council
- Rontgen-Angstrom Cluster
- European Regional Development Fund [CZ.02.1.01/0.0/0.0/16_019/0000789, CZ.02.1.01/0.0/0.0/15_003/0000447]
- Swedish Foundation for Strategic Research
- Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
- Wellcome Trust [204732/Z/16/Z]
- Ministry of Education, Youth and Sports
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-76SF00515]
- Wellcome Trust [204732/Z/16/Z] Funding Source: Wellcome Trust
The Flash X-ray Imaging (FXI) technique, under development at X-ray free electron lasers (XFEL), aims to achieve structure determination based on diffraction from individual macromolecular complexes. We report an FXI study on the first protein complex-RNA polymerase II-ever injected at an XFEL. A successful 3D reconstruction requires a high number of observations of the sample in various orientations. The measured diffraction signal for many shots can be comparable to background. Here we present a robust and highly sensitive hit-identification method based on automated modeling of beamline background through photon statistics. It can operate at controlled false positive hit-rate of 3 x10(-5). We demonstrate its power in determining particle hits and validate our findings against an independent hit-identification approach based on ion time-of-flight spectra. We also validate the advantages of our method over simpler hit-identification schemes via tests on other samples and using computer simulations, showing a doubled hit-identification power.
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