3.8 Article

1H, 15N, and 13C Resonance Assignments of the Anti-CRISPR AcrIIA4 from Listeria monocytogenes Prophages

期刊

出版社

KOREAN MAGNETIC RESONANCE SOC
DOI: 10.6564/JKMRS.2018.22.3.071

关键词

AcrIIA4; anti-CRISPR; Cas9; NMR spectroscopy

资金

  1. Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration [PJ013181]
  2. Creative-Pioneering Researchers Program through Seoul National University [500-20180204]

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The CRISPR-Cas system is the adaptive immune system in bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, an endonuclease Cas9 cleaves DNA targets of phages as directed by guide RNA comprising crRNA and tracrRNA. To avoid targeting and destruction by Cas9, phages employ anti-CRISPR (Acr) proteins that act against host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone H-1, N-15, and( 13)C resonance assignments of AcrIIA4 that inhibits endonuclease activity of type II-A Listeria monocytogenes Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The secondary structures of AcrIIA4 predicted by the backbone chemical shifts show an alpha beta beta beta alpha alpha fold, which is used to determine the solution structure.

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