期刊
JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY
卷 22, 期 3, 页码 71-75出版社
KOREAN MAGNETIC RESONANCE SOC
DOI: 10.6564/JKMRS.2018.22.3.071
关键词
AcrIIA4; anti-CRISPR; Cas9; NMR spectroscopy
资金
- Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration [PJ013181]
- Creative-Pioneering Researchers Program through Seoul National University [500-20180204]
The CRISPR-Cas system is the adaptive immune system in bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, an endonuclease Cas9 cleaves DNA targets of phages as directed by guide RNA comprising crRNA and tracrRNA. To avoid targeting and destruction by Cas9, phages employ anti-CRISPR (Acr) proteins that act against host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone H-1, N-15, and( 13)C resonance assignments of AcrIIA4 that inhibits endonuclease activity of type II-A Listeria monocytogenes Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The secondary structures of AcrIIA4 predicted by the backbone chemical shifts show an alpha beta beta beta alpha alpha fold, which is used to determine the solution structure.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据