期刊
NEW PHYTOLOGIST
卷 213, 期 4, 页码 1632-1641出版社
WILEY
DOI: 10.1111/nph.14443
关键词
biotechnology; cassava (Manihot esculenta); food security; friable embryogenic callus; gene expression; organized embryogenic structures; RNA sequencing
资金
- Bill and Melinda Gates Foundation
- NCI Cancer Center Support Grant from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) [P30 CA91842]
- ICTS/CTSA Grant from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) [UL1 TR000448]
- NIH Roadmap for Medical Research
Cassava (Manihot esculenta) feeds c. 800 million people world-wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA-sequencing and develop an open access, web-based interface for further interrogation of the data. Through this dataset, we consider the physiology of cassava. Specifically, we focus on identification of the transcriptional signatures that define the massive, underground storage roots used as a food source and the favored target tissue for transgene integration and genome editing, friable embryogenic callus (FEC). Further, we identify promoters able to drive strong expression in multiple tissue/organs. The information gained from this study is of value for both conventional and biotechnological improvement programs.
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