期刊
NEW PHYTOLOGIST
卷 214, 期 2, 页码 655-667出版社
WILEY
DOI: 10.1111/nph.14414
关键词
Arabidopsis thaliana; carbon concentrating mechanism (CCM); Chlamydomonas reinhardtii; chloroplast; photosynthesis; pyrenoid; Rubisco; tobacco
资金
- UK Biotechnology and Biological Sciences Research Council (Institute Strategic Programme Grant) [BB/J004561/1, BB/I024453/1, BB/M006468/1]
- John Innes Foundation
- Lancaster Environment Centre
- BBSRC [BB/M006352/1, BB/M006468/1, BB/I024453/1, BB/M007693/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M006468/1, BB/M007693/1, BBS/E/J/00000020, BB/M006352/1, BB/I024453/1] Funding Source: researchfish
Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency. Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU alpha-helices. Leaves of Arabidopsis rbcs mutants expressing 'pyrenoid-competent' chimeric Arabidopsis SSUs containing the SSU alpha-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the alpha-helices are catalytically neutral. The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.
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