期刊
NEW JOURNAL OF CHEMISTRY
卷 41, 期 1, 页码 377-386出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nj02890j
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资金
- National Research Foundation of Korea's (NRF) - Korean Government (MEST) [NRF-2016R1A2B4012488]
- National Creative Research Initiative Programs of the National Research Foundation of Korea (NRF) - Korean Government (MSIP) [2012R1A3A2048814]
Two new dinuclear iridium (Ir) complexes bridged by a conjugated aromatic tppz ligand, (bhq)(2)Ir(tppz)Ir(bhq)(2) (1) and (ppy)(2)Ir(tppz)Ir(ppy)(2) (2) (bhq = benzo(h)quinolone, ppy = phenyl-pyridine, tppz = tetrapyrido[ 3,2-a:2',3'-c:3 '',2 ''-h:2''',3'''-j] phenazine), were prepared. The DNA binding properties of complexes 1 and 2 were studied using single-stranded DNA, double-stranded DNA, and G-quadruplex DNA. G-Quadruplex binding was characterized by higher emission enhancements than those observed with other control DNA strands. Photophysical properties of complexes 1 and 2 indicated that 1 displayed selective G-quadruplex binding affinity, but 2 did not. The emission properties of complexes 1 and 2 were also studied using liposomes in order to understand their possible interactions with lipid-rich organelles in live cells. The phosphorescence intensity of 1 and 2 was enhanced upon interaction with liposomes, but 2 showed greater enhancement. We also confirmed that 1 and 2 could be used as probes in live cells. Complexes 1 and 2 were intensely stained in HeLa cervical cancer cells. Excitation with a lambda = 488 nm laser showed red emission localized in the cytosol of cells. Cellular studies showed that 1 and 2 were located inside HeLa cells, but neither complex passed through the nuclear membrane. However, complexes 1 and 2 possessed superior photostability, indicating their role as good luminescent agents for imaging and tracking the endoplasmic reticulum (ER) in live cells. Overall, complex 1 exhibited better selectivity for G-quadruplexes, liposomes, and ER staining.
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