期刊
NEW JOURNAL OF CHEMISTRY
卷 41, 期 17, 页码 9230-9235出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nj02384g
关键词
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资金
- National Natural Science Foundation of China [81572081, 81273480, 21175070]
In this paper, a novel turn-on fluorescence aptasensor has been designed for adenosine detection based on fluorescence resonance energy transfer (FRET) from single-stranded DNA labeled carbon dots (ssDNA-CDs) to aptamer modified gold nanoparticles (aptamer-AuNPs). In the absence of adenosine, the fluorescence of ssDNA-CDs was quenched by aptamer-AuNPs via the formation of an aptamerss-DNA duplex. The introduced adenosine competed to displace ssDNA-CDs by specifically binding to the aptamer, resulting in the recovery of the quenched fluorescence of ssDNA-CDs. Under optimized conditions, the increase in fluorescence intensity was proportional to the concentration of adenosine with a linear range of 10-500 nM and allowed a limit of detection as low as 4.2 nM. In addition, this method was successfully applied to adenosine determination in human serum samples with satisfactory results.
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