期刊
ACS OMEGA
卷 3, 期 12, 页码 17164-17172出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.8b02918
关键词
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资金
- FSU
- National Science Foundation (NSF-CHE) [1508501, 1058957]
- National Institutes of Health (NIH) [R01 DC013080]
- Asahi-Kasei Corp.
- Intramural Research Program of the National Cancer Institute (National Institutes of Health) [BC011313]
The steady progress made over the past three decades in growing a variety of inorganic nanomaterials, with discreet control over their size and photophysical properties, has been exploited to develop several imaging and sensing applications. However, full integration of these materials into biology has been hampered by the complexity of delivering them into cells. In this report, we demonstrate the effectiveness of a chemically synthesized anticancer peptide to facilitate the rapid delivery of luminescent quantum dots (QDs) into live cells. We combine fluorescence imaging microscopy, flow cytometry, and specific endocytosis inhibition experiments to probe QD-peptide conjugate uptake by different cell lines. We consistently find that a sizable fraction of the internalized conjugates does not co-localize with endosomes or the nuclei. These findings are extremely promising for the potential integration of various nanomaterials into biological systems.
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