期刊
NEUROSCIENCE
卷 340, 期 -, 页码 521-529出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2016.11.006
关键词
angiotensin II AT(1) receptors; amphetamine sensitization; c-Fos/TH immunoreactivity; locus coeruleus; nucleus of the solitary tract; blood pressure response
资金
- CONICET [11220120100373CO-KB1]
- SECyT
- FonCyT PRESTAMO BID [PICT 2011-2476]
A single exposure to amphetamine induces neurochemical sensitization in striatal areas. The neuropeptide angiotensin II, through AT(1) receptors (AT(1)-R) activation, is involved in these responses. However, amphetamine induced alterations can be extended to extra-striatal areas involved in blood pressure control and their physiological outcomes. Our aim for the present study was to analyze the possible role for AT(1)-R in these events using a two injection protocol and to further characterize the proposed AT(1)-R antagonism protocol. Central effect of orally administered AT(1)-R blocker (Candesartan, 3 mg/kg p.o. x 5 days) in male Wistar rats was analyzed by spontaneous activity of neurons within locus coeruleus. In another group of animals pretreated with the AT(1)-R blocker or vehicle, sensitization was achieved by a single administration of amphetamine (5 mg/kg i.p.- day 6) followed by a 3-week period off drug. On day 27, after receiving an amphetamine challenge (0.5 mg/kg i.p.), we evaluated: (1) the sensitized c-Fos expression in locus coeruleus (LC), nucleus of the solitary tract (NTS), caudal ventrolateral medulla (A1) and central amygdala (CeAmy); and (2) the blood pressure response. AT(1)-R blockade decreased LC neurons' spontaneous firing rate. Moreover, sensitized c-Fos immunoreactivity in TH + neurons was found in LC and NTS; and both responses were blunted by the AT(1)-R blocker pretreatment. Meanwhile, no differences were found neither in CeAmy nor Al. Sensitized blood pressure response was observed as sustained changes in mean arterial pressure and was effectively prevented by AT(1)-R blockade. Our results extend AT1-R role in amphetamine-induced sensitization over noradrenergic nuclei and their cardiovascular output. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据