期刊
NEUROREPORT
卷 28, 期 18, 页码 1255-1260出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0000000000000909
关键词
absence epilepsy; development; GABA(A) receptors; stargazer mouse; thalamocortical network; western blotting
资金
- University of Otago
- Roche New Zealand for Hanns Mohler Doctoral Scholarship
- Dean's Bequest Fund
- UORG grant
Absence seizures are known to originate from disruptions within the corticothalamocortical network; however, the precise underlying cellular and molecular mechanisms that induce hypersynchronicity and hyperexcitability are debated and likely to be complex and multifactorial. Recent studies implicate impaired thalamic GABAergic inhibition as a common feature in multiple animal models of absence epilepsy, including the well-established stargazer mouse model. Recently, we demonstrated region-specific increases in the whole tissue and synaptic levels of GABA(A) receptor (GABA(A)R) subunits 1 and 2, within the ventral posterior region of the thalamus in adult epileptic stargazer mice compared with nonepileptic control littermates. The objective of this study was to investigate whether such changes in GABA(A)R subunits 1 and 2 can be observed before the initiation of seizures, which occur around postnatal (PN) days 16-18 in stargazers. Semiquantitative western blotting was used to analyze the relative tissue level expression of GABA(A)R 1 and 2 subunits in the thalamus of juvenile stargazer mice compared with their nonepileptic control littermates at three different time points before the initiation of seizures. We show that there is a statistically significant increase in the expression of 1 and 2 subunits in the thalamus of stargazer mice, at the PN7-9 stage, compared with the control littermates, but not at PN10-12 and PN13-15 stages. These results suggest that an aberrant expression of GABA(A)R subunits 1 and 2 in the stargazers does not occur immediately before seizure onset and therefore is unlikely to directly contribute to the initiation of absence seizures.
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