期刊
NEUROPHARMACOLOGY
卷 118, 期 -, 页码 148-156出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2017.03.009
关键词
Acetazolamide; D-phenylalanine; 1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-Trimethylpyridiniumperchlorate
资金
- University of Firenze funds
- FAPESP research fellowship [Proc. 2012/05838-0]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq Brazil) [201511/2014-2]
- Fondazione Umberto Veronesi
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/05838-0] Funding Source: FAPESP
Rats injected with by D-phenylalanine, a carbonic anhydrase (CA) activator, enhanced spatial learning, whereas rats given acetazolamide, a CA inhibitor, exhibited impairments of fear memory consolidation. However, the related mechanisms are unclear. We investigated if CAs are involved in a non-spatial recognition memory task assessed using the object recognition test (ORT). Systemic administration of acetazolamide to male CD1 mice caused amnesia in the ORT and reduced CA activity in brain homogenates, while treatment with D-phenylalanine enhanced memory and increased CA activity. We provided also the first evidence that D-phenylalanine administration rapidly activated extracellular signal regulated kinase (ERK) pathways, a critical step for memory formation, in the cortex and the hippo campus, two brain areas involved in memory processing. Effects elicited by D-phenylalanine were completely blunted by co-administration of acetazolamide, but not of 1-N-(4-sulfamoylphenyl-ethyl)2,4,6-trimethylpyridinium perchlorate ((C18).) a CA inhibitor that, differently from acetazolamide, does not cross the blood brain barrier. Our results strongly suggest that brain but not peripheral CAs activation potentiates memory as a result of ERK pathway enhanced activation. (C) 2017 Elsevier Ltd. All rights reserved.
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