期刊
EMBO REPORTS
卷 16, 期 7, 页码 836-850出版社
WILEY-BLACKWELL
DOI: 10.15252/embr.201439937
关键词
Atrx; Daxx; heterochromatin; histone H3.3; IAP retrotransposons
资金
- Deutsche Forschungsgemeinschaft [SFB 1064, SPP1356]
- ERC grant LatentCauses
More than 50% of mammalian genomes consist of retrotransposon sequences. Silencing of retrotransposons by heterochromatin is essential to ensure genomic stability and transcriptional integrity. Here, we identified a short sequence element in intracisternal A particle (IAP) retrotransposons that is sufficient to trigger heterochromatin formation. We used this sequence in a genome-wide shRNA screen and identified the chromatin remodeler Atrx as a novel regulator of IAP silencing. Atrx binds to IAP elements and is necessary for efficient heterochromatin formation. In addition, Atrx facilitates a robust and largely inaccessible heterochromatin structure as Atrx knockout cells display increased chromatin accessibility at retrotransposons and non-repetitive heterochromatic loci. In summary, we demonstrate a direct role of Atrx in the establishment and robust maintenance of heterochromatin.
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