期刊
SEPARATIONS
卷 5, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/separations5040048
关键词
HILIC; amino acids; silica monolithic column
资金
- Czech Science Foundation [16-03749S]
- Ministry of the Interior of the Czech Republic [VI20172020069]
- Czech Academy of Sciences [RVO:68081715]
A strategy for the preparation of silica-based monolithic capillary columns (150 x 0.1 mm) with high selectivity to amino acids is presented. The zwitterionic columns were prepared by coating the silica monolith with [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. The columns were evaluated under isocratic conditions in hydrophilic interaction liquid chromatography. The best separation of amino acids was obtained on the monolithic column prepared by a stepwise modification procedure where the modification step was repeated four times. The mixture of fifteen amino acids was separated within 13 min using the mobile phase consisting of 75% acetonitrile and 25% 5 mmol/L ammonium acetate at pH 4.5.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据