期刊
NEUROLOGICAL RESEARCH
卷 40, 期 2, 页码 117-121出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/01616412.2017.1398882
关键词
ECV304; blood-brain barrier; permeability; tightness; monoculture
资金
- Youth Foundation of Beijing Tiantan Hospital [2016-YQN-11]
Objective: ECV304/C6 co-culture model is a widely used tool for BBB studies. However, cell source may influence the establishment of co-culture model and some C6 cells could damage the barrier integrity. Here, we established an ECV304 monoculture model and evaluated it in the respect of tightness, tight junction proteins and discriminative brain penetration. Methods: The tightness of ECV304 cell layers was evaluated by the measurement of permeability to hydrophilic marker Lucifer yellow. Immunofluorescence method was explored to detect the expression of tight junction proteins occludin, claudin-5 and ZO-1 in ECV304 cells. The discriminative brain penetration of the model was assessed by a permeability testing of compounds with different penetration rates, including digoxin, quinidine, and propranolol. Results: The ECV304 monolayers developed low permeability to Lucifer yellow (permeability coefficient: 0.31 +/- 0.02 x 10(-3) cm/min) and exhibited positive immunostaining of occludin, claudin-5 and ZO-1. The permeability coefficients of high permeable quinidine and propranolol across ECV304 cell layers were higher than that of low permeable digoxin by 3.6 and 2.8-fold, respectively. Conclusions: The ECV304 monoculture model developed tight paracellular barrier and discriminated between compounds with different permeability, indicating it as a potential in vitro model for BBB permeability assessment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据